LETHEEN-AGAR BASE MODIFIED

General description

GranuCult^® plus Letheen Agar Base is used for the microbiological sampling of environmental surfaces that are treated with disinfectants. It is a highly nutritious medium containing Lecithin and Tween^® 80 for neutralizing quaternary ammonium compounds contained in sanitizers. Beef extract and tryptone provide nitrogen, carbon, sulfur, and other essential growth factors. Glucose is the source of energy, and agar is the solidifying agent. The media is a modification of the AOAC formulae.

Application

GranuCult^® plus Letheen Agar is used to neutralize quaternary ammonium compounds, for sampling of environmental surfaces that have been treated with disinfectants.

Features and Benefits

• GranuCult^® offers superior granulated culture media.
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment.
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions.
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): turbid
Appearance (colour): yellowish-brown
pH-value (25 °C): 7.0 - 7.4
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): good to very good
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): good to very good
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): good to very good
Growth (Staphylococcus epidermidis ATCC 12228 (WDCM 00036)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Incubation: 24 - 48 hrs.; 35 °C; aerobic.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Agency: according to FDA-BAM. Quality Level: 200. form: medium granules (dehydrated (DCM)). packaging: pkg of 500 . g. manufacturer/tradename: GranuCult^®. plus. storage condition: protect from light (Keep tightly closed and dry). technique(s): microbiological culture: suitable. color: beige. pH: 7.0-7.4 (25 . °C, 52.1 . g/L in H₂O, after autoclaving). solubility: 52.1 . g/L. application(s): cosmetics, microbiology. storage temp.: 15-25°C. suitability: Enterococcus spp., Escherichia coli, Pseudomonas spp., Salmonella spp., Staphylococcus spp.. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3.