Lee's HiVeg Agar, 5KG

Lee's HiVegTM agar is used for differential enumerations of yoghurt starter bacteria ( Lactobacillus bulgaricus and Streptococcus thermophilus ).This medium described by Lee et al is used for the differential enumeration of yoghurt starter bacteria. This medium is also recommended by APHA for the same purpose. It contains sucrose, which most L. bulgaricus strains will not ferment, but S. thermophilus will, and lactose, which both species utilize. Bromocresol purple is the pH indicator, which turns yellow in acidic condition and imparts yellow color to the colony. Lactobacillus bulgaricus shows luxuriant growth on media with white colored colony; while Streptococcus thermophilus grows luxuriantlly giving yellow colored colony. This medium is prepared by replacing animal peptones with vegetable defined peptones to avoid BSE/TSE/GMO risk. Application: For Laboratory Chemicals, Analytical Purpose, Biochemical Analysis Appearance of powder: Light grey colored may have slightly greenish tinge homogeneous, free flowing powder Gelling: Firm, comparable with 1.8% Agar gel Color and Clarity: Purple colored, opaque gel forms in petri plates Reaction: Reaction of 5.15% w/v aqueous solution is pH 7.0 ± 0.2 at 25°C Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 48 hours in presence of Carbon dioxide (CO2)