LECTIN FROM PISUM SATIVUM-FITC LABELED

General description

Lectin from Pisum sativum (pea) is a tetrameric glycoprotein of about 48 kDa. It is isolated from peas. Pisum sativum agglutinin is specific for α-D-glucose and α-D-mannose.

Application

Lectin from Pisum sativum (pea) has been used to determine acrosomal status and viability of spermatozoa and to assess sperm acrosome reaction (AR) patterns

Biochem/physiol Actions

Lectin from Pisum sativum (pea) possess anti-hepatoma effect and mitogenicity to lymphocytes, like concanavalin A (Con A).

PSA is not blood group specific, but has an affinity for terminal α-D-mannosyl residues. PSA is a mitogen similar to concanavalin A.

Preparation Note

Conjugates are prepared from affinity purified lectin.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

conjugate: FITC conjugate. Quality Level: 200. form: lyophilized powder. potency: <. 20 . μ. g per mL agglutination activity. composition: Protein, ≥. 95% biuret . extent of labeling: 2-4 . mol FITC per mol protein (Lowry).. impurities: salt, free. storage temp.: −. 20°C. Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H317 - H334. Precautionary Statements: P261 - P280 - P342 + P311. Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Faceshields, Gloves.