General Western Blot Protocol:Glycoprotein sample size: 500ngLectin Concentration: 0.1ug/mlLoad samples at 500 ng of glycoprotein per laneRun 4-20% Bis-Tris SDS page gelTransfer gel to a PVDF membraneBlock membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RTWash membrane 5 x 5 minutes with 25ml RIPA bufferDetect using chemiluminescent substrate (CPS1-120)
Specification: Isolectin B4 (BSI-B4), peroxidase conjugate, lyophilized powder
- UPC:
- 10161641
- Condition:
- New
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- L755047-0.5mg
- Product Size:
- 0.5mg
akash.verma@cenmed.com
(732) 447-1115





