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l929/r

L929/R

Catalog No.
C005B-243690
Manufacturer No.
04102001-1VL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
VL

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Cell Line Origin Mouse C3H/An connective tissue cisplatin resistant Cell Line Description This cisplatin-resistant cell line has been developed by exposure of the parent L929 murine fibroblast cell line (Sigma Catalogue number. 85011425) to

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Cell Line Origin

Mouse C3H/An connective tissue cisplatin resistant

Cell Line Description

This cisplatin-resistant cell line has been developed by exposure of the parent L929 murine fibroblast cell line (Sigma Catalogue number. 85011425) to increasing concentrations of cisplatin in vitro. L929/R cells can be used in the development of novel anti-cancer treatments. The parent cell line L929 was derived from normal subcutaneous areolar adipose tissue.

Application

Development of novel anti-cancer treatments

Culture Medium

RPMI 1640 + 2mM L-Glutamine + 10% Foetal Bovine Serum (FBS) + 0.26μM Cisplatin.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:50 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 2% CO2; 37 C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

UPC:
41106402
Condition:
New
HazmatClass:
No
MPN:
04102001-1VL
Temperature Control Device:
Yes

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