L-GLUTAMINE (C005B-049346)

Biochem/physiol Actions

L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Analysis Note

Assay (perchloric acid titration, calculated on dried substance): 99.0 - 101.0 %
Identity (IR-spectrum): passes test
Appearance: white to almost white, fine powder
Appearance of solution (25 g/l; CO₂-free water): clear and colorless
Spec. rotation (α 20/D, 100 g/l, hydrochloric acid 2 mol/l, calc. on dried substance): +31,5 to +33,0
Chloride (Cl): ≤ 200 ppm
Sulfate (SO₄): ≤ 200 ppm
Heavy metals (as Pb): ≤ 10 ppm
As (Arsenic): ≤ 5 ppm
Ca (Calcium): ≤ 10 ppm
Co (Cobalt): ≤ 5 ppm
Fe (Iron): ≤ 5 ppm
K (Potassium): ≤ 5 ppm
Mg (Magnesium): ≤ 5 ppm
Na (Sodium): ≤ 50 ppm
Zn (Zinc): ≤ 5 ppm
Ninhydrin-positive substances (LC)(any ninhydrin-positive impurity): ≤ 0.3 %
Ninhydrin-positive substances (LC) (ammonium (570 nm)): ≤ 0.1 %
Ninhydrin-positive substances (LC)(total impurities): ≤ 0.5 %
Loss on drying (105 °C; 3 h): ≤ 0.3 %

Molecular Weight: 146.14. Empirical Formula: C₅H₁₀N₂O₃. linearFormula: H₂NCOCH₂CH₂CH(NH₂)CO₂H. Quality Level: 100. form: solid. potency: 7500 . mg/kg LD₅₀, oral (Rat). pH: 4.0-6.0 (20 . °C, 25 . g/L in H₂O). mp: 185 . °C (dec.) (lit.). solubility: 100 . g/L. density: 1.47 . g/cm³ . at 20 . °C. bulk density: 640 . kg/m³. storage temp.: 2-30°C. SMILES string: N[C@@H](CCC(N): O)C(O): O. InChI: 1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1. InChI key: ZDXPYRJPNDTMRX-VKHMYHEASA-N. Storage Class Code: 13 - Non Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.