KOVAC'S REAGENT FOR INDOLES FOR MICROBIOLOGY (C005B-002680)

General description

Kovac′s reagent for indoles is a solution of 4-(dimethylamino)benzaldehyde and hydrochloric acid in n-butanol.

Application

Kovac′s reagent for indoles has been used for enumerating E. coli O157:H7 in ground beef samples in different scenarios of stored, thawed, and as cooked patty samples. It has also been used for E coli enumeration in dewatered blackwater.

Biochem/physiol Actions

Kovac′s reagent detects the indole and aids in identifying the indole-positive and indole-negative microorganisms. E. coli can split tryptophan into indole and α-aminopropionic acid in the presence of the enzyme tryptophanase. p-Aminobenzaldehyde present in the reagent reacts with indole to form a cherry-red complex, that is soluble in alcohol, ether, and chloroform. It is recommended to use glucose free media and peptone with a high tryptophan content for the test.

Molecular Weight: 149.19. Empirical Formula: C₉H₁₁NO. shelf life: limited shelf life, expiry date on the label. Quality Level: 100. composition: n-butanol , 4-dimethylaminobenzaldehyde , hydrochloric acid . technique(s): microbe id | specific enzyme detection: suitable. refractive index: n20/D 1.417. application(s): agriculture
clinical testing
environmental
food and beverages, microbiology. storage temp.: 2-8°C. suitability: Escherichia coli, coliforms. SMILES string: [H]C(: O)c1ccc(cc1)N(C)C. InChI: 1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3. InChI key: BGNGWHSBYQYVRX-UHFFFAOYSA-N. Pictograms: GHS02,GHS05,GHS07. Signal Word: Danger. Hazard Statements: H226 - H290 - H302 - H315 - H318 - H335 - H336. Precautionary Statements: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2. Storage Class Code: 3 - Flammable liquids. WGK: WGK 1. Flash Point(F): 96.8 °F. Flash Point(C): 36 °C. Personal Protective Equipment: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter.