KARPAS 45

Cell Line Origin

Human T-cell Leukaemia

Cell Line Description

A human T cell leukaemia cell line established from the bone marrow of a 2-year-old boy diagnosed with acute lymphoblastic leukaemia (ALL) prior to therapy in 1972; cells were reported to carry the MLL-AFX and AFX-MLL fusion genes, the original diagnosis was T-ALL.

Culture Medium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS.

Subculture Routine

Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO₂; 37°C. Doubling time 50-100 hours. Split saturated culture 1:2 to 1:3 every 2-3 days. Spherical cells growing in clusters in suspension.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.