KARPAS-25

Cell Line Origin

Human acute plasma cell leukaemia

Cell Line Description

Established in 1989 from the pleural effusion of a 27-year old woman diagnosed with acute plasma cell leukaemia who failed to respond to treatment and died within 3 weeks.

Culture Medium

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Subculture Routine

Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 10⁵ cells/ml; 5% CO₂; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 10⁵ cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 10⁵ cells/ml, this tends to equate to a 1:3 split every 2-3 days.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.