JHAF (C005B-235282)

General description

Consanguineous: Yes

Homozygous: Yes

Cell Line Description

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA Profile

A: 31012
Cw: 1502
B: 5101
HLA DRB1: 407
HLA DRB4: 103
HLA DQA1: 301
HLA DQB1: 301
HLA DPA1: 1
HLA DPB1: 301

Serological Profile

HLA A: 31
HLA A ief: 31
HLA B: 51
HLA B ief: 51
Bw4/bw6: 4
HLA Dw: 13
HLA DR: 4
HLA DR53: 53
HLA DQ: 7
HLA DP: 3

Culture Medium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subculture Routine

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

biological source: human blood. ethnicity: English. gender: male. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblastoid. technique(s): cell culture | mammalian: suitable. shipped in: dry ice.