INVERTASE ACTIVITY ASSAY KIT

General description

Invertase (β-fructofuranosidase) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertase is produced by certain plants, microorganisms, and honey bees.

Application

Invertase Assay Kit has been used for screening inhibition of recombinant invertase inhibitor protein from sugarcane.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of invertase activity in biological and environmental samples and for the screening of invertase inhibitors.

Principle

In this kit, invertase activity is determined by a coupled enzyme assay in which invertase cleaves sucrose to glucose and fructose, resulting in a colorimetric (570 nm)/fluorometric (λ_ex = 535/λ_em = 587 nm) product, proportional to the invertase activity present. One unit of Invertase is the amount of enzyme that will catalyze the formation of 1.0 μmole of glucose per minute at pH 4.5 under the assay conditions.