INTERLEUKIN-8 HUMAN RECOMBINANT EXPRESSED IN E. COLI

Application

Interleukin-8 (IL-8) human has ben used to:

• measure neutrophil chemotoxis
• study the uptake of apoptotic cells by meningothelial cells
• study the effect of IL-8 on expression and secretion of urocortin-1 by human umbilical vein endothelial cells

Biochem/physiol Actions

Interleukin-8 (IL-8) was formerly called monocyte-derived neutrophil chemotactic factor. It belongs to the chemokine α or C-X-C family. Like other members of this family, the mature form of IL-8 has four cysteine residues and the first two cysteine residues are separated by glutamine. IL-8 shows chemotactic activity in vitro for T cells, neutrophils and basophils. Mutation in IL-8 can increase risk to acute pancreatitis.

Interleukin-8 (IL-8), formerly called monocyte-derived neutrophil chemotactic factor, belongs to the chemokine α or C-X-C family. The mature form of IL-8 has 4 cysteine residues, as do the other members of the chemokine family and the first two cysteine residues are separated by glutamine. Mature human IL-8 consists of 72 amino acids with a molecular mass of 8.4 kDa. IL-8 exhibits chemotactic activity in vitro for T cells, basophils, as measured by enzymes including myeloperoxidase, α-mannosidase and β-glucuronidase. IL-8 is an angiogenic factor.

Physical form

Lyophilized from a 0.2 μm filtered solution with no additives.

Analysis Note

The biological activity is determined by its ability to chemoattract human peripheral blood neutrophils using a concentration range of 10.0-100.0 ng/ml.

biological source: human. Quality Level: 100. recombinant: expressed in E. coli. Assay: ≥. 98% (SDS-PAGE and HPLC). form: lyophilized powder. mol wt: 8.4 . kDa. packaging: pkg of 10 . μ. g. technique(s): cell culture | mammalian: suitable. impurities: endotoxin, tested. UniProt accession no.: P10145. storage temp.: −. 20°C. Gene Information: human ... IL8(3576). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).