INTERLEUKIN-1?ETA HUMAN IL-1?ETA RECOMBINANT EXPRESSED IN HEK 293 CELLS HUMANKINE(R) SUITABLE FOR CELL CULTURE CELL CULTURE TE

Biochem/physiol Actions

Interleukin-1 (IL-1), originally known as Lymphocyte Activating Factor (LAF), activates T cell lymphocytes, which then proliferate and secrete interleukin-2 (IL-2). IL-1 is released primarily from stimulated macrophages and monocytes, but has also been shown to be released from several other types and is thought to play a key role in inflammatory and immune responses. Other synonyms for IL-1 include: endogenous pyrogen (EP), mitogenic protein (MP), Helper Peak-1 (HP-1), T Cell Replacing Factor III (TRF III or TRFM), B Cell Activating Factor (BAF) and B Cell Differentiation Factor (BDF) The two closely related agents Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) share 62% homology in amino acid sequence and elicit nearly identical biological responses. IL-1α and IL-1β are both approximately 17 kDa with some heterogeneity in the amount of glycosylation.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Preparation Note

HumanKine Recombinant Human IL-1β, expressed in human HEK 293 cells, is a glycosylated monomer with a molecular mass of 18-25 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The activity is determined by the dose-dependent stimulation of the proliferation of mouse D10S cells.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

biological source: human. Quality Level: 100. recombinant: expressed in HEK 293 cells. Assay: ≥. 95% (SDS-PAGE). form: lyophilized powder. potency: ≤. 0.05 . ng/mL EC₅₀. quality: endotoxin tested. mol wt: dimer 18-25 . kDa (glycosylated). packaging: pkg of 10 . μ. g. technique(s): cell culture | mammalian: suitable. impurities: <. 1 . EU/μ. g. UniProt accession no.: P01584. storage temp.: −. 20°C. Gene Information: human ... IL1B(3553). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.