INSULIN-LIKE GROWTH FACTOR-I RECEPTOR RECOMBINANT HUMAN

General description

IGF1R (insulin-like growth factor I) is a receptor tyrosine kinase. The gene is mapped to human chromosome 15q26. It is expressed in various solid tumors as well as hematologic malignancies.

Application

Insulin-like growth factor-I receptor human has been used to study the binding constants of estradiol to insulin, insulin receptors and insulin-like peptides.

Biochem/physiol Actions

IGF-I receptor, a tyrosine kinase receptor with signal transduction pathways, is comprised of two α and two β subunits. IGF-I receptor binds IGF-I with high affinity, IGF-II with lower affinity, and insulin with weak affinity.

Physical form

Lyophilized from a 0.2 μm solution in phosphate buffered saline.

Analysis Note

As a result of glycosylation in SDS-PAGE under reducing conditions, the recombinant α subunit migrates as a doublet of ~120 kDa and 150 kDa and the β subunit migrates as doublet of ~46 kDa and 50 kDa.

The biological activity is measured by its ability to bind human IGF­-­I in a functional ELISA assay.

biological source: human. Quality Level: 200. recombinant: expressed in NSO cells. Assay: ≥. 95% (SDS-PAGE). form: lyophilized powder. mol wt: α. -chain ~120 &. 150 . kDa, β. subunit ~46 &. 50 . kDa. packaging: pkg of 50 . μ. g. impurities: endotoxin, tested. UniProt accession no.: P08069. storage temp.: −. 20°C. Gene Information: human ... IGF1R(3480). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).