INSOLUTION AKT INHIBITOR X - CAS 925681-41-0 - CALBIOCHEM

General description

A cell-permeable, reversible, and selective inhibitor of the phosphorylation of Akt and its in vitro kinase activity (complete inhibition <5 M) with minimal effect on PI 3-K, PDK1, or SGK1. Shown to suppress growth of Rh (rhabdomyosarcoma) cell lines (IC50 = 2-5 M), inhibit IGF-I-stimulated nuclear translocation of Akt, and prevent phosphorylation of the downstream targets, mTOR, p70S6 kinase, and S6 ribosomal protein. Unlike Akti1/2, the mode of inhibition is not PH domain-dependent. Also shown to induce neuronal autophagy in an Akt- and mTOR-independent manner and enhances the clearance of misfolded protein. The solid form of this compound is also available.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Akt

Reversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Physical form

A 20 mM (2 mg/262 µL) solution of Akt Inhibitor X (Cat. No. 124020) in H₂O.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C.

Other Notes

Tsvetkov, A.S., et al. 2010. Proc. Natl. Acad. Sci. USA107, 16982.
Thimmaiah, K.N., et al. 2005. J. Biol. Chem.280, 31924.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Molecular Weight: 344.88 (free base basis). Empirical Formula: C₂₀H₂₅ClN₂O · xHCl. Quality Level: 100. Assay: ≥. 97% (HPLC). form: liquid. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles, protect from light. shipped in: dry ice. storage temp.: −. 70°C. SMILES string: ClC(C: C1): CC2: C1OC3: C(N2CCCCN(CC)CC)C: CC: C3. InChI: 1S/C20H25ClN2O.ClH/c1-3-22(4-2)13-7-8-14-23-17-9-5-6-10-19(17)24-20-12-11-16(21)15-18(20)23. /h5-6,9-12,15H,3-4,7-8,13-14H2,1-2H3. 1H. InChI key: SVKSJUIYYCQZEC-UHFFFAOYSA-N. Storage Class Code: 12 - Non Combustible Liquids. WGK: nwg. Flash Point(F): Not applicable. Flash Point(C): Not applicable.