IMPRINT ULTRA CHROMATIN OPTIMIZATION KIT

General description

The Imprint Ultra Chromatin Optimization Kit provides reagents needed to optimize chromatin sonication/shearing conditions before performing ChIP. It is imperative that chromatin is completely sheared into fragments with a peak around 500 base pairs (bp) for ChIP-qPCR assays, and < 500 bp for ChIP-Seq and ChIP-chip, for maximum specificity and resolution, and to ensure low background. For higher resolution applications like genome-wide location analysis by ChIP-chip or ChIP-Seq, sonication of input chromatin to below 500 bp, with a peak around 250 bp, is critical.

Components

Sufficient reagents are provided to perform three sets of chromatin sonication optimization experiments. Each set would include five different conditions for sonication to optimize the parameters. Reagents are provided for a total of 15 reactions, including preparation of cell lysates from 10⁷ cells/reaction, nuclei lysis, sonication, cross-link reversal and DNA purification.

Legal Information

Imprint is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level: 200. Pictograms: GHS02,GHS03,GHS05,GHS07,GHS08,GHS09. Signal Word: Danger. Hazard Statements: H226 - H271 - H290 - H302 - H314 - H334 - H336 - H410. Precautionary Statements: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3. Target Organs: Central nervous system. Storage Class Code: 5.1A - Strongly oxidizing hazardous materials. WGK: WGK 3. Flash Point(F): 100.4 °F. Flash Point(C): 38 °C.