Imidazole, Reagent, 100g

Catalog No.: C003B-523266
Manufacturer No.: 16905
Manufacturer Name: ELECTRON MICROSCOPY SCIENCES
Quantity: 1
Unit of Measure: EA

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Description
Specifications

Imidazole is a liquid enhancing buffering agent that, when used with 4% osmium tetroxide solution as a post-fixation for tissue blocks, produces a prominent electron-opaque staining of lipid droplets with no evidence of diffusion. Procedure: Tissue is fixed by vascular perfusion with 1.5% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) containing 4% polyvinyl pyrolidone and 0.05% CaCl₂. Sections about 50um thick are cut and placed in 0.2M Imidazole in which 4% aqueous OsO₄ is added. The final concentration of OsO₄ is 2% and the pH is 7.5. Note that the tissue must first be placed in Imidazole buffer; then the OsO₄ is added. This post-fixation is carried out for 30 minutes at room temperature. Grade: Reagent CAS #: 288-32-4

UPC:: 51101906
HazmatClass:: No
WeightUOM:: LB
MPN:: 16905

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