IMIDAZOLE BIOULTRA >= 99.5 % GC (C005B-029590)

Application

Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase and as chelator for the binding of various divalent cations. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for detection of proteins.

Excellent for buffers in the range of pH 6.2-7.8

Other Notes

Buffer for assay of horseradish peroxidase

Molecular Weight: 68.08. Empirical Formula: C₃H₄N₂. vapor pressure: <. 1 mmHg ( 20 °C). Quality Level: 200. product line: BioUltra. Assay: ≥. 99.5% (GC). form: powder or crystals. impurities: insoluble matter, passes filter test, ≤. 0.2% water. ign. residue (900 °C): ≤. 0.05%. pH: 9.5-11.0 (25 . °C, 0.1 . M in H₂O). pKa (25 °C): 6.95. bp: 256 . °C (lit.). mp: 88-91 . °C (lit.). solubility: H₂O: 0.1 . M at . 20 . °C, clear, colorless. anion traces: chloride (Cl^-): ≤. 50 . mg/kg, sulfate (SO₄^2-): ≤. 50 . mg/kg. cation traces: Al: ≤. 5 . mg/kg, As: ≤. 0.1 . mg/kg, Ba: ≤. 5 . mg/kg, Bi: ≤. 5 . mg/kg, Ca: ≤. 10 . mg/kg, Cd: ≤. 5 . mg/kg, Co: ≤. 5 . mg/kg, Cr: ≤. 5 . mg/kg, Cu: ≤. 5 . mg/kg, Fe: ≤. 5 . mg/kg, K: ≤. 50 . mg/kg, Li: ≤. 5 . mg/kg, Mg: ≤. 5 . mg/kg, Mn: ≤. 5 . mg/kg, Mo: ≤. 5 . mg/kg, Na: ≤. 50 . mg/kg, Ni: ≤. 5 . mg/kg, Pb: ≤. 5 . mg/kg, Sr: ≤. 5 . mg/kg, Zn: ≤. 5 . mg/kg. λ: 0.1 . M in H₂O. UV absorption: λ. : 260 . nm A_max: 0.10, λ. : 280 . nm A_max: 0.10. SMILES string: c1c[nH]cn1. InChI: 1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5). InChI key: RAXXELZNTBOGNW-UHFFFAOYSA-N. Pictograms: GHS05,GHS07,GHS08. Signal Word: Danger. Hazard Statements: H302 - H314 - H360D. Precautionary Statements: P260 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C. Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects. WGK: WGK 2. Flash Point(F): 293.0 °F - closed cup. Flash Point(C): 145 °C - closed cup. Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges.