IMIDAZOLE BUFFER SOLUTION BIOULTRA 1M WATER (C005B-029597)

General description

Imidazole is used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for the assay of horseradish peroxidase and as a chelator for the binding of various divalent cations. Imidazole aids the elution of histidine-containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for the detection of proteins.

Application

Imidazole buffer solution has been used as a component:

• of calmodulin-binding buffer 1 (CBB1) for the tandem affinity purification (TAP) of TAP-tagged proteins together with co-purifying proteins
• of lysis buffer, and SDS loading buffer for His-ubiquitin pull-down assays
• of imidazole wash and elution buffer for protein purification

Molecular Weight: 68.08. Empirical Formula: C₃H₄N₂. product line: BioUltra. Quality Level: 200. form: liquid. concentration: 1 . M in H₂O. impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected. pH: 9.5±0.5. density: 1.01 . g/mL . at 20 . °C. SMILES string: c1c[nH]cn1. InChI: 1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5). InChI key: RAXXELZNTBOGNW-UHFFFAOYSA-N. Pictograms: GHS05,GHS08. Signal Word: Danger. Hazard Statements: H314 - H360D. Precautionary Statements: P201 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P305 + P351 + P338 + P310 - P308 + P313. Hazard Classifications: Eye Dam. 1 - Repr. 1B - Skin Corr. 1C. Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter.