IGEPAL CA 630 (C005B-087961)

General description

IGEPAL^® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40.

Application

IGEPAL^® CA-630 has been used:

• In phosducin co-precipitation assay for pull down of Epitope-Tagged ^HAG_γ1 and ^HAG_γ1C71S
• As a component of lysis buffer for cell culture and sample preparation for MAPK (mitogen-activated protein kinases) analysis, Immunoblotting
• In the extraction of cells in Oil Red O staining procedure to visualise lipid formation in cells during adipogenesis
• As a component of lysis buffer containing Tris-HCl, NaCl and EDTA for immunoblotting experiment

Other Notes

Nonionic, non-denaturing detergent

Quality

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Caution

May become turbid on storage; a clear product can be obtained on heating to 40°C.

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

Legal Information

IGEPAL is a registered trademark of Solvay

Nonidet is a trademark of Shell Chemical Co.

linearFormula: (C₂H₄O)_nC₁₄H₂₂O. description: non-ionic. Quality Level: 100. form: liquid. mol wt: ~603.0 . g/mol. refractive index: n20/D 1.492. CMC: 0.08 . mM (20-25°C). transition temp: cloud point 63-67  . °C. density: 1.06 . g/mL . at 20 . °C. anion traces: chloride (Cl^-): ≤. 50 . mg/kg, sulfate (SO₄^2-): ≤. 100 . mg/kg. cation traces: Ca: ≤. 10 . mg/kg, Cd: ≤. 5 . mg/kg, Co: ≤. 5 . mg/kg, Cr: ≤. 5 . mg/kg, Cu: ≤. 5 . mg/kg, Fe: ≤. 5 . mg/kg, K: ≤. 1000 . mg/kg, Mg: ≤. 5 . mg/kg, Mn: ≤. 5 . mg/kg, Na: ≤. 1000 . mg/kg, Ni: ≤. 5 . mg/kg, Pb: ≤. 5 . mg/kg, Zn: ≤. 5 . mg/kg. HLB: 13. InChI: 1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3. InChI key: JYCQQPHGFMYQCF-UHFFFAOYSA-N. Pictograms: GHS05,GHS07,GHS09. Signal Word: Danger. Hazard Statements: H302 - H315 - H318 - H410. Precautionary Statements: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): 483.8 °F. Flash Point(C): 251 °C. Personal Protective Equipment: Eyeshields, Gloves, type ABEK (EN14387) respirator filter.