The rAmylase Project: Using Ion Exchange Chromatography to Separate Proteins, 8 x 4 student groups

This kit is designed to teach 8 groups of four students the principles of Ion Exchange Chromatography and protein purification. The kit contains the reagents to allow students to: Equilibrate a DEAE agarose column Load a protein sample on the column (amylase, lysozyme or a mixture of both) Wash the unbound protein sample from the column Bind and then elute the bound protein sample from the column with a salt buffer Measure the amount of eluted protein with a Bradford protein assay Generate an elution profile determining which protein blinded to the column and which flowed through Give evidence that the two proteins were separated by the chromatography technology Features For 8 groups of 4 students Requires micropipettes and tips Optional requirement for a spectrophotometer Applications HS-LS1: From Molecules to Organisms: Structures and Processes LS1.A: Structure and Function