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ht29-mtx-e12

HT29-MTX-E12

Catalog No.
C005B-094939
Manufacturer No.
12040401-1VL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
VI

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Cell Line Origin HT29, HT29-MTX, methotrexate, mucous layer, intestinal permeation, oral drug absorption Cell Line Description HT29 cells were differentiated into mature goblet cells using methotrexate. Mucous-secreting HT29-MTX subclones were

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Cell Line Origin

HT29, HT29-MTX, methotrexate, mucous layer, intestinal permeation, oral drug absorption

Cell Line Description

HT29 cells were differentiated into mature goblet cells using methotrexate. Mucous-secreting HT29-MTX subclones were isolated from this cell clone and characterized with regard to tight junction formation, development of confluent monolayers and productio

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 17

Culture Medium

DMEM + 2mM Glutamine + 1% Non essential amino acids + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split subconfluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin/EDTA; 7.5-10% CO2; 37 °C. Suggested seeding density 2-4x10,000 cells/cm².

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source: human colon. growth mode: Adherent. karyotype: Not specified. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.
UPC:
41101801
Condition:
New
HazmatClass:
No
MPN:
12040401-1VL
Temperature Control Device:
Yes

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