HMG-1

General description

HMG-1 (high mobility group protein) is a nuclear protein of molecular weight 30kDa. It is secreted from macrophages, monocytes and necrotic cells. It is ubiquitously expressed. The protein has basic HMG (high mobility group) box domains and an acidic carboxyl-terminus.

Application

HMG-1 (high-mobility-group protein) human has been used:

• to prepare a standard curve for measuring HMG1 using ELISA (enzyme-linked immunosorbent assay) test
• to study the apoptotic ratios of Lewis cells in presence of exogenous HMG-1
• to study the changes in Th (T helper)-17 cell expansion in the presence of HMG-1 under in vitro conditions
• for the migration assay of GM7373 cells

Biochem/physiol Actions

HMG-1 (high mobility group protein) is a non-histone chromosomal protein, which promotes the sequence specific DNA attachment of proteins. HMG1 binds DNA in a non-sequence specific manner. It is involved in DNA replication, transcription and repair. In addition, it is up-regulated in cancers and is associated with cellular growth, vasculogenesis and apoptosis regulation. In the presence of infection or injury, HMG1 is secreted and mediates inflammation. It is associated with the pathogenesis of autoimmune diseases, such as rheumatoid arthritis (RA), inflammatory bowel disease (IBD) and systemic lupus erythematosus (SLE).

Physical form

Lyophilized powder containing HEPES buffer salts, pH 7.8, NaCl, EDTA, Triton X-100, Leupeptin and AEBSF.

Analysis Note

Tested in DNA retardation assay

recombinant: expressed in E. coli. Quality Level: 200. Assay: ≥. 90% (SDS-PAGE). form: lyophilized powder. mol wt: 25 . kDa (215 amino acids. comprises two homologous HMG boxes, rich in basic amino acids, and an acidic tail at the carboxy-terminus). UniProt accession no.: P09429. shipped in: dry ice. storage temp.: −. 20°C. SMILES string: COC(: O)CCCCCCCCCCCCCCO. Gene Information: human ... HMGB1(3146). Pictograms: GHS05,GHS09. Signal Word: Danger. Hazard Statements: H318 - H410. Precautionary Statements: P273 - P280 - P305 + P351 + P338 - P391 - P501. Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves.