HISTRAP FAST FLOW 5 X 5 ML (C005B-183439)

General description

HisTrap^™ FF is a ready-to-use column, prepacked with precharged Ni Sepharose^™ 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose^™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

The high flow rates made possible by the Ni Sepharose^™ 6 Fast Flow matrix make HisTrap^™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap^® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

Features and Benefits

• High binding capacity, approx. 40 mg/mL medium.
• Negligible leakage of Ni²⁺.
• Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva

HisTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

shelf life: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.. packaging: pack of 5 × 5 . mL. manufacturer/tradename: Cytiva 17-5255-01. parameter: <. 4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.), 42 psi. bed size: 7 . mm × 25 . mm . bed volume: 1 . mL . column I.D.: 7 . mm . matrix: 6% cross-linked agarose. particle size: 45-165 . μ. m. average diameter: 90 . μ. m. cleaning: 2-14(Ni²⁺-stripped medium.). working range: 3-12(Ni²⁺-stripped medium.). capacity: ~40 . mg binding capacity(histidine-tagged protein). suitability: suitable for bioprocess medium. Pictograms: GHS09. Signal Word: Warning. Hazard Statements: H226. Precautionary Statements: P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501. Storage Class Code: 3 - Flammable liquids.