HISTOPAQUE 1083 (C005B-093520)

General description

Histopaque^®-1083 is a solution containing polysucrose and sodium diatrizoate, adjusted to a density of 1.083 g/mL. It facilitates the recovery of viable mononuclear cells from small volumes of blood of rats, mice, and other small mammals.

Application

Histopaque^®-1083 has been used for the isolation of mononuclear cells and leukocytes from rat peripheral blood.

Features and Benefits

Histopaque^®-1083 offers numerous advantages, such as:

• Optimal recovery of viable cells
• Permits selective separation of blood cell types
• Prevents cell distortion
• Preserves cell viability
• Minimal extraneous cell interference
• Reproducible performance from batch to batch
• Sterile-filtered and endotoxin-tested
• Stable at 2-8°C for at least 2 years when protected from light

Principle

Anticoagulated blood is layered onto HISTOPAQUE1083. During centrifugation, erythrocytes areaggregated by polysucrose and rapidly sediment;whereas, lymphocytes and other mononuclear cells remain at the plasma-HISTOPAQUE 1083 interface. Erythrocytes pellet to the bottom of the centrifuge tube.

Legal Information

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

sterility: sterile-filtered. Quality Level: 400. shelf life: Expiry date on the label.. impurities: endotoxin, tested. density: 1.083 . g/mL. application(s): hematology
histology. storage temp.: 2-8°C. Pictograms: GHS05. Signal Word: Warning. Hazard Statements: H290 - H315 - H319. Precautionary Statements: P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313. Hazard Classifications: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2. Storage Class Code: 8A - Combustible, corrosive hazardous materials. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter.