HISTOPAQUE-1077 HYBRI-MAX (C005B-200777)

General description

Histopaque^®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm³. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)² typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.

Application

Histopaque^®-1077 Hybri-Max^™ has been used to isolate:

• peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation
• immune cells (lymphocytes) from peripheral blood
• PBMCs from blood

Legal Information

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

sterility: sterile-filtered. Quality Level: 400. product line: BioReagent. form: liquid. technique(s): cell culture | hybridoma: suitable, single cell analysis: suitable. impurities: endotoxin, tested. density: 1.077 . g/cm³ . at 25 . °C (lit.). web metadata keyword.default: density gradient, gradient centrifugation, lymphocyte purification reagent. storage temp.: 2-8°C. Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H317 - H334. Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313. Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter.