HEPES BIOXTRA PH 5.0-6.5 (20 ¢€“'C 1 M IN H2O) (C005B-059373)

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:

• As a component of homogenisation buffer used in protein sample preparation
• To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation
• As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation
• As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

Molecular Weight: 238.30. Empirical Formula: C₈H₁₈N₂O₄S. product line: BioXtra. Quality Level: 400. Assay: ≥. 99.5% (titration). form: powder. impurities: Insoluble matter, passes filter test. ign. residue (900 °C): ≤. 0.1% (as SO₄). loss: ≤. 1.0% loss on drying, 110°C. pH: 5.0-6.5 (1 . M in H₂O). useful pH range: 6.8-8.2. pKa (25 °C): 7.5. solubility: H₂O: 1 . M, clear, colorless. anion traces: chloride (Cl^-): ≤. 0.05%. cation traces: Al: ≤. 0.0005%, As: ≤. 0.0001%, Ba: ≤. 0.0005%, Bi: ≤. 0.0005%, Ca: ≤. 0.001%, Cd: ≤. 0.0005%, Co: ≤. 0.0005%, Cr: ≤. 0.0005%, Cu: ≤. 0.0005%, Fe: ≤. 0.0005%, K: ≤. 0.005%, Li: ≤. 0.0005%, Mg: ≤. 0.0005%, Mn: ≤. 0.0005%, Mo: ≤. 0.0005%, Na: ≤. 0.005%, Ni: ≤. 0.0005%, Pb: ≤. 0.0005%, Sr: ≤. 0.0005%, Zn: ≤. 0.0005%. absorption: ≤. 0.035 at 280 in H₂O at 1 . M, ≤. 0.045 at 260 in H₂O at 1 . M. application(s): diagnostic assay manufacturing. SMILES string: OCCN1CCN(CC1)CCS(O)(: O): O. InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14). InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N. Storage Class Code: 13 - Non Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).