HEPES >= 99.5% (TITRATION) BIOXTRA SUITABLE FOR MOUSE EMBRYO CELL CULTURE (C005B-059369)

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. It is typically used in cell culture at concentration between 5mM to 30mM.

Application

HEPES has been used:

• As a constituent of modified Eagle′s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells
• As a component of modified Ham′s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production
• in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation

Other Notes

This product has been qualified for use in cell and mouse embryo culture.

Easily compare specifications for HEPES products with the HEPES specification table.

Physical properties

Useful pH range: 6.8 - 8.2

Molecular Weight: 238.30. Empirical Formula: C₈H₁₈N₂O₄S. product line: BioXtra. Quality Level: 400. Assay: ≥. 99.5% (titration). form: powder. technique(s): cell culture | embryo: suitable. pH: 5.0-6.5 (25 . °C, 238 . g/L). useful pH range: 6.8-8.2. pKa (25 °C): 7.5, 7.5. solubility: water: 500 . mg/mL, clear, colorless. absorption: ≤. 0.05 at 290 in H₂O at 33%. SMILES string: OCCN1CCN(CC1)CCS(O)(: O): O. InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14). InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).