HEPATOCYTE GROWTH FACTOR HUMAN HGF RECOMBINANT EXPRESSED IN BACULOVIRUS INFECTED HIGH-5 CELLS SUITABLE FOR CELL CULTURE (C005B-200578)

Biochem/physiol Actions

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Other Notes

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

Physical form

Lyophilized from a 0.2 μm filtered solution in 20 mM NaH₂PO₄ and 500 mM NaCl, pH 7.0 with 50 μg BSA per 1 μg HGF

Preparation Note

Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. After reconstitution, this solution can be stored at 2-8 °C for up to one month or at -20 °C to -70 °C for up to three months. Us a manual defrost freezer and avoid repeated freeze-thaw cycles.

Analysis Note

The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osieosarcoma cells.

biological source: human. Quality Level: 200. recombinant: expressed in Baculovirus infected High-5 cells. Assay: >. 95% (SDS-PAGE). form: lyophilized powder. potency: 1.0-20.0 . ng/mL. quality: endotoxin tested. mol wt: ~80 . kDa. packaging: pkg of 5x5 . μ. g, pkg of 5 . μ. g. storage condition: avoid repeated freeze/thaw cycles. technique(s): cell culture | mammalian: suitable. impurities: <. 1.0 . EU/μ. g. UniProt accession no.: P14210. storage temp.: −. 20°C. Gene Information: human ... HGF(3082). Storage Class Code: 11 - Combustible Solids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves.