HEPARIN-BINDING EGF-LIKE GROWTH FACTOR HUMAN HB-EGF RECOMBINANT EXPRESSED IN E. COLI POWDER SUITABLE FOR CELL CULTURE (C005B-114038)

Biochem/physiol Actions

Heparin-binding epidermal growth factor (HB-EGF), like BTC, is a heparin-binding EGF family member. Natural soluble HB-EGF is a 19 kDa glycoprotein of multiple forms due to heterogeneous truncations. Soluble HB-EGF is mitogenic and chemotactic for fibroblasts, keratinocytes, and smooth muscle cells but not capillary endothelial cells. Production of HB-EGF is detected in monocytes, macrophages, and vascular and aortic smooth muscle cells (SMC). HB-EGF may play a role in the pathogenesis of atherosclerosis. It may also be an effector of Jun-induced oncogenic transformation. HB-EGF binds to EGFR with high affinity and to ErbB with moderate affinity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in the EGF-responsive BALB/3T3 cells.

biological source: human. Quality Level: 100. recombinant: expressed in baculovirus infected Sf21 cells. Assay: ≥. 97% (SDS-PAGE and N-terminal analysis). form: lyophilized powder. potency: ≤. 3 . ng/mL. quality: endotoxin tested. mol wt: 9.5 . kDa. packaging: pkg of 50 and 250 . μ. g. storage condition: avoid repeated freeze/thaw cycles. technique(s): cell culture | mammalian: suitable. web metadata keyword.default: BTC, blastocyst, implantation. UniProt accession no.: Q99075. storage temp.: −. 20°C. Gene Information: human ... HBEGF(1839), HBEGF(1839). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).