GUANIDINE THIOCYANATE BIOREAGENT FOR MOLECULAR BIOLOGY >=99% (C005B-050867)

Application

Guanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis. It can be used for deactivation of endogenous RNases leading to denaturation of nucleoprotein complexes. It is also suitable for denaturation of macromolecules such as nucleic acids and proteins and for RNA isolation.

Chaotropic agent and strong denaturant; solubilizes cells.

Biochem/physiol Actions

Guanidine thiocyanate (GTC) plays a role in protein denaturation. It is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures ribonuclease (RNase). GTC has been studied in the unfolding and refolding of fluorescent proteins (FP). It can be used along with phenol and chloroform for extraction of RNA.

Principle

Guanidine thiocyanate interferes with the hydrogen bonds and intramolecular interactions of macromolecules. It solubilizes the proteins resulting in disintegration of cellular structures.

Molecular Weight: 118.16. Empirical Formula: CH₅N₃ · CHNS. linearFormula: NH₂C(: NH)NH₂ · HSCN. biological source: synthetic. Quality Level: 200. grade: for molecular biology. product line: BioReagent. Assay: ≥. 99%. form: powder. technique(s): RNA extraction: suitable. impurities: DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected. color: colorless to white,

. mp: 115-122 . °C, 120-122 . °C (lit.). solubility: H₂O: 4 . M at . 20 . °C, clear, colorless. anion traces: chloride (Cl^-): ≤. 5000 . mg/kg. cation traces: Al: ≤. 5 . mg/kg, As: ≤. 0.1 . mg/kg, Ba: ≤. 5 . mg/kg, Bi: ≤. 5 . mg/kg, Ca: ≤. 10 . mg/kg, Cd: ≤. 5 . mg/kg, Co: ≤. 5 . mg/kg, Cr: ≤. 5 . mg/kg, Cu: ≤. 5 . mg/kg, Fe: ≤. 5 . mg/kg, K: ≤. 50 . mg/kg, Li: ≤. 5 . mg/kg, Mg: ≤. 5 . mg/kg, Mn: ≤. 5 . mg/kg, Mo: ≤. 5 . mg/kg, Na: ≤. 5000 . mg/kg, Ni: ≤. 5 . mg/kg, Pb: ≤. 5 . mg/kg, Sr: ≤. 5 . mg/kg, Zn: ≤. 5 . mg/kg. storage temp.: room temp. SMILES string: SC#N.NC(N): N. InChI: 1S/CH5N3.CHNS/c2-1(3)4. 2-1-3/h(H5,2,3,4). 3H. InChI key: ZJYYHGLJYGJLLN-UHFFFAOYSA-N. Pictograms: GHS05,GHS07. Signal Word: Danger. Hazard Statements: H302 + H312 + H332 - H314 - H412. Precautionary Statements: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C. Supp Hazards: EUH032. Storage Class Code: 8B - Non-combustible, corrosive hazardous materials. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves.