GUANIDINE HYDROCHLORIDE SOLUTION BIOULTRA ~ 8M WATER (C005B-044760)

Application

Guanidine hydrochloride solution has been used as a fixation buffer in genomic DNA extraction for genomic analysis.

Biochem/physiol Actions

Guanidine‐HCl (Gnd-HCl), an efficient chaotropic agent, is broadly used in RNA isolation, globular protein denaturation, and protein refolding studies as a denaturant. It is an appropriate sample buffer for the quick and effective elution of affinity-purified protein complexes and the subsequent protein digestion step. Gnd-HCl is not preferred in proteomics experiments due to its hindrance with trypsin activity at low molar concentrations and tendency to precipitate when combined with sodium dodecyl sulfate (SDS). GnHCl is commonly utilized to extract proteins in extracellular matrix (ECM)‐rich tissues including bone, cartilage, ligament, and tendon.

Molecular Weight: 95.53. Empirical Formula: CH₅N₃ · HCl. product line: BioUltra. Quality Level: 100. form: liquid. concentration: ~8 . M in H₂O. impurities: insoluble matter, passes filter test. refractive index: n20/D 1.465. pH: 4.5-7.5 (25 . °C). anion traces: sulfate (SO₄^2-): ≤. 50 . mg/kg. cation traces: Al: ≤. 1 . mg/kg, As: ≤. 0.1 . mg/kg, Ba: ≤. 1 . mg/kg, Bi: ≤. 1 . mg/kg, Ca: ≤. 5 . mg/kg, Cd: ≤. 1 . mg/kg, Co: ≤. 1 . mg/kg, Cr: ≤. 1 . mg/kg, Cu: ≤. 1 . mg/kg, Fe: ≤. 1 . mg/kg, K: ≤. 20 . mg/kg, Li: ≤. 1 . mg/kg, Mg: ≤. 1 . mg/kg, Mn: ≤. 1 . mg/kg, Mo: ≤. 1 . mg/kg, Na: ≤. 60 . mg/kg, Ni: ≤. 1 . mg/kg, Pb: ≤. 1 . mg/kg, Sr: ≤. 1 . mg/kg, Zn: ≤. 1 . mg/kg. λ: neat. UV absorption: λ. : 260 . nm A_max: 0.040, λ. : 280 . nm A_max: 0.025. SMILES string: Cl.NC(N): N. InChI: 1S/CH5N3.ClH/c2-1(3)4. /h(H5,2,3,4). 1H. InChI key: PJJJBBJSCAKJQF-UHFFFAOYSA-N. Gene Information: human ... KCNA1(3736), KCNA10(3744), KCNA2(3737), KCNA3(3738), KCNA4(3739), KCNA5(3741), KCNA6(3742), KCNA7(3743), KCNB1(3745), KCNB2(9312), KCNC1(3746), KCNC2(3747), KCNC3(3748), KCNC4(3749), KCND1(3750), KCND2(3751), KCND3(3752), KCNF1(3754), KCNG1(3755), KCNG2(26251), KCNG3(170850), KCNG4(93107), KCNH1(3756), KCNH2(3757), KCNH3(23416), KCNH4(23415), KCNH5(27133), KCNH6(81033), KCNH7(90134), KCNH8(131096), KCNQ1(3784), KCNQ2(3785), KCNQ3(3786), KCNQ4(9132), KCNQ5(56479), KCNS1(3787), KCNS2(3788), KCNS3(3790), KCNV1(27012), KCNV2(169522). Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H302 + H332 - H315 - H319. Precautionary Statements: P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter.