GUANIDINE HYDROCHLORIDE >= 99.0% (AT) (C005B-044763)

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Molecular Weight: 95.53. Empirical Formula: CH₅N₃ · HCl. linearFormula: NH₂C(: NH)NH₂ · HCl. Quality Level: 200. Assay: ≥. 99.0% (AT). form: solid. ign. residue: ≤. 0.1% (as SO₄). pH: (25 . °C, 4.6 - 6/573 . g/L). mp: 180-185 . °C (lit.). solubility: H₂O: 6 . M at . 20 . °C, clear, colorless. density: 1.3 . g/cm³ (lit.). anion traces: sulfate (SO₄^2-): ≤. 50 . mg/kg. cation traces: Ca: ≤. 20 . mg/kg, Cd: ≤. 5 . mg/kg, Co: ≤. 5 . mg/kg, Cr: ≤. 5 . mg/kg, Cu: ≤. 5 . mg/kg, Fe: ≤. 5 . mg/kg, K: ≤. 50 . mg/kg, Mg: ≤. 5 . mg/kg, Mn: ≤. 5 . mg/kg, Na: ≤. 50 . mg/kg, Ni: ≤. 5 . mg/kg, Pb: ≤. 5 . mg/kg, Zn: ≤. 5 . mg/kg. SMILES string: Cl[H].NC(N): N. InChI: 1S/CH5N3.ClH/c2-1(3)4. /h(H5,2,3,4). 1H. InChI key: PJJJBBJSCAKJQF-UHFFFAOYSA-N. Gene Information: human ... KCNA1(3736), KCNA10(3744), KCNA2(3737), KCNA3(3738), KCNA4(3739), KCNA5(3741), KCNA6(3742), KCNA7(3743), KCNB1(3745), KCNB2(9312), KCNC1(3746), KCNC2(3747), KCNC3(3748), KCNC4(3749), KCND1(3750), KCND2(3751), KCND3(3752), KCNF1(3754), KCNG1(3755), KCNG2(26251), KCNG3(170850), KCNG4(93107), KCNH1(3756), KCNH2(3757), KCNH3(23416), KCNH4(23415), KCNH5(27133), KCNH6(81033), KCNH7(90134), KCNH8(131096), KCNQ1(3784), KCNQ2(3785), KCNQ3(3786), KCNQ4(9132), KCNQ5(56479), KCNS1(3787), KCNS2(3788), KCNS3(3790), KCNV1(27012), KCNV2(169522). Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H302 + H332 - H315 - H319. Precautionary Statements: P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Faceshields, Gloves.