Cell Line Origin
Fish Grunt fin tissue blue striped
Cell Line Description
Derived from fin tissue of the adult salt water blue striped Grunt. Does not support the replication of influenza A, herpes simplex, adeno, polio, dengue and eastern equine encephalitis viruses.
Culture Medium
EMEM (HBSS) (Sigma cat no. M5775) + 10mM Hepes (pH 7.3) + 2mM Glutamine + 0.23% (w/v) NaHCO3 + 20% FBS (Heat Inactivated)+ 58mM NaCl
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 20°C. After resuscitation split cells 1:2 to 1:4 and culture for at least one week with 1-2 media changes before shipping. Plating efficiency can be very low a
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: fish fin. growth mode: Adherent. karyotype: Modal no. 47. morphology: Fibroblast-like. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 10121702
- Condition:
- New
- HazmatClass:
- No
- MPN:
- 88010601-1VL
- Temperature Control Device:
- Yes
akash.verma@cenmed.com
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