GRAM'S DECOLORIZING SOLUTION FOR THE GRA (C005B-093890)

General description

Gram′s decolorizing solution - for the Gram staining method, is a ready-to-use solution used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram′s crystal violet solution (Product number 1.09218) - an aniline dye - in the first step. After the treatment with iodine solution (Lugol′s solution stabilized with PVP, Product number 1.00567), a dye-iodine complex will form. During the decolorizing step (Gram′s decolorizing solution, Product number 1.10218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram′s safranin solution (Gram′s safranine solution (Product number 1.09217) and appear pink to red.
A bottle with 500 ml Gram′s decolorizing solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Product number 1.11885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU).

Analysis Note

Suitability for microscopy: passes test
Staphylococcus aureus: dark blue-violet
E.coli: pink to red

Quality Level: 400. form: liquid. autoignition temp.: >. 425 . °C. IVD: for in vitro diagnostic use. transition temp: flash point -10 . °C. density: 0.79 . g/cm³ . at 20 . °C. application(s): clinical testing
diagnostic assay manufacturing
hematology
histology. storage temp.: 15-25°C. Pictograms: GHS02,GHS07. Signal Word: Danger. Hazard Statements: H225 - H319 - H336. Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338. Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3. Target Organs: Central nervous system. Storage Class Code: 3 - Flammable liquids. WGK: WGK 1. Flash Point(F): 14.0 °F. Flash Point(C): -10 °C.