GLYCOLYSIS ASSAY KIT

General description

Glycolysis is one of the major metabolic pathways cells undergo to produce energy and results in the production of pyruvate. One of the eventual end products of this process is lactate dehydrogenase-mediated conversion of pyruvate to L-lactate via lactic acid fermentation. This enzymatic mechanism allows L-lactate to serve as an indicator of glycolysis.

Features and Benefits

• Linear detection range up to 10 mM L-lactate in 96-well plate assay.
• Convenient. The procedure is performed at room temperature and involves adding a single working reagent.
• Compatible with high-throughput handling systems.

Suitability

The kit is suitable for the direct determination of L-Lactate produced by glycolysis in cell media samples and for the screening of glycolysis inhibitors.

Principle

The Glycolysis Assay Kit is based on measuring the production of L-Lactate from glycolysis in cells. L-Lactate that is secreted into the cell media is quantified using a coupled reaction involving the lactate dehydrogenase catalyzed oxidation of L-lactate that generates pyruvate and NADH, which subsequently reduces a formazan dye. The intensity of the reduced dye, measured at 565 nm, is directly proportional to the L-lactate concentration in the sample, which in turn is directly proportional to the glycolytic rate of the cells.