Application
Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.
Analysis Note
| Acrolein, glucose, and ammonium compounds | To pass test |
| Assay | 95% min |
| Chloride (Cl) | 0.001% max |
| Chlorinated compounds (as Cl) | 0.003% max |
| Color | To pass test |
| Description | To pass test |
| Fatty acids and esters | To pass test |
| Heavy metals (as Pb) | 5ppm max |
| Identification | To pass test |
| Readily carbonizable substances | To pass test |
| Residue after ignition | 0.01% max |
| Specific Gravity at 25/25°C | 1.249 min |
| Sulfate (SO4) | 0.002% max |
Molecular Weight: 92.09. Empirical Formula: C
3H
8O
3. linearFormula: HOCH
2CH(OH)CH
2OH. vapor density: 3.1 (vs air). Quality Level: 200. vapor pressure: <. 1 mmHg ( 20 °C). Assay: ≥. 95%. form: liquid. autoignition temp.: 698 . °F. technique(s): electrophoresis: suitable. impurities: ≤. 0.001% Chloride (Cl), ≤. 0.002% Sulfate (SO4), ≤. 0.003% Chlorinated compounds (as Cl), ≤. 5 . ppm Heavy metals (as Pb). refractive index:
n20/D 1.474 (lit.). pH: 5 (20 . °C, 100 . g/L in H
2O). bp: 182 . °C/20 . mmHg (lit.). mp: 20 . °C (lit.). transition temp: flash point 199 . °C. density: 1.25 . g/mL (lit.). shipped in: ambient. storage temp.: room temp. SMILES string: OCC(O)CO. InChI: 1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2. InChI key: PEDCQBHIVMGVHV-UHFFFAOYSA-N. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): 390.2 °F - Pensky-Martens closed cup. Flash Point(C): 199 °C - Pensky-Martens closed cup.