GLUTAMINE AND GLUTAMATE DETERMINATION KIT

Application

For enzymatic determination of L-glutamine and L-glutamate via reduction of NAD to NADH, measured spectrophotometrically at 339 nm.
The detection ranges for this kit are: 0.056 - 0.56 mM L-glutamate; 0.14 - 1.4 mM L-glutamine.

Glutamine and Glutamate Determination Kit has been used to determine the concentration of L-glutamine:

• in minimum essential medium (MEM) medium
• in the supernatant
• in plasma

Biochem/physiol Actions

The Glutamine/Glutamate Determination Kit is designed for the spectrophotometric measurement of L-glutamine and/or L-glutamate via enzymatic deamination of L-glutamine and dehydrogenation of L-glutamate with conversion of NAD⁺ to NADH. This kit may be used for the spectrophotometric measurement of L-glutamine and/or L-glutamate in liquid preparations.

usage:  . kit sufficient for 50 . tests. Quality Level: 200. packaging: pkg of 1 . kit. storage condition: dry at room temperature. technique(s): UV/Vis spectroscopy: suitable. λ. _max: 340 nm. web metadata keyword.default: Glutamine quantitation, glutamate quantitation. application(s): cell analysis
detection. storage temp.: 2-8°C. Pictograms: GHS02,GHS05,GHS06,GHS08,GHS09. Signal Word: Danger. Hazard Statements: H226 - H301 + H311 - H314 - H317 - H330 - H350 - H410. Precautionary Statements: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1B - Skin Sens. 1. Storage Class Code: 3 - Flammable liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.