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glucose dehydrogenase assay kit

GLUCOSE DEHYDROGENASE ASSAY KIT

Catalog No.
C005B-447996
Manufacturer No.
MAK450-1KT
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
KT

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General description Glucose Dehydrogenase (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway.

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General description

Glucose Dehydrogenase (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway.

Features and Benefits

  • Linear detection range (20 μL sample): 0.5 to 200 U/L for 15 minute reaction.
  • Compatible with high-throughput handling systems.

Suitability

The kit is suitable for the quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism in biological samples (e.g. plasma, serum, tissue and culture media).

Principle

The Glucose Dehydrogenase Activity Assay Kit is a non-radioactive, colorimetric GDH assay. It is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A565 nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.

UPC:
41115830
Condition:
New
HazmatClass:
No
MPN:
MAK450-1KT
Temperature Control Device:
Yes

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