GLIAL CELL LINE -DERIVED NEUROTROPHIC FACTOR RAT RECOMBINANT EXPRESSED IN SF21 CELLS

Biochem/physiol Actions

Glial cell-derived neurotrophic factor (GDNF) is a neurotrophic factor that is a member of the TGF-β superfamily. GDNF is founding member of the GDNF family of ligands, which to date include GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). GDNF is a glycosylated disulfide-linked homodimeric protein of ~15 kDa. Mature rat and human GDNF share 93% sequence homology with strong species cross-reactivity. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development including motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. In addition, exogenously applied GDNF has been shown to rescue damaged facial motor neurons in vivo.

Physical form

Lyophilized from 50 μL of a 0.2 um filtered solution in PBS, pH 7.4 with 50 ug BSA per 1 ug as a carrier protein

Analysis Note

The biological activity of GDNF is measured by its ability to bind to immobilized rrGFRa1/Fc in a functional ELISA.

biological source: rat. Quality Level: 200. recombinant: expressed in baculovirus infected Sf21 cells. Assay: ≥. 97% (SDS-PAGE). form: lyophilized powder. potency: 0.10-10 . ng/mL. mol wt: ~30 . kDa. packaging: pkg of 10 . μ. g. storage condition: avoid repeated freeze/thaw cycles. technique(s): cell culture | mammalian: suitable. impurities: endotoxin, tested. UniProt accession no.: Q07731. storage temp.: −. 20°C. Gene Information: rat ... Gdnf(25453). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).