This antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Detection of Gli-3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-3 is likely to be low. Furthermore, Gli3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.
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- 41116158
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 600-401-694
- Temperature Control Device:
- Yes
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