GENELUTE 96 WELL TOTAL RNA PURIFICATION KIT

General description

GenElute 96 Well Total RNA Purification Kit allows for the high-throughput purification of total RNA from cells or tissue. The kit utilizes the advantages of silica membrane technology and eliminates the use of hazardous organic compounds such as phenol and chloroform.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The purified total RNA is ready for use in any enzymatic downstream application.

Features and Benefits

• Unique plate design offers low risk of cross-contamination
• RNA can be used for any kind of downstream enzymatic reaction
• Processing possible under with a vacuum or centrifuge
• Suitable for manual and automated processing

Principle

Sigma′s GenElute 96 Well Total RNA Purification Kit provides a simple and convenient way to isolate total RNA from cells or tissue. The cells or tissue are lysed in a solution containing large amounts of chaotropic salt which immediately inactivates RNases that are present in almost all biological materials. The addition of a Wash Buffer provides the necessary binding conditions for the RNA to be adsorbed to the silica membrane. The contaminating DNA which may be bound to the silica membrane is removed by directly applying RNase-free DNase. The remaining salts, proteins, and other cellular debris are removed by additional washing steps. The final step is to elute the purified RNA in the RNAase-free water that is supplied in the kit.

Other Notes

Application notes:
Method of purification:Silica-membrane technology
Compatible with Manual or automated, vacuum or centrifugation
Efficient recovery of > 200 nt
Typical yield20 μg (2 x 106 HeLa cells),
20 μg (20 mg mouse liver)
Typical concentration50–200 ng/μL
Elution volume50–130 μL
Preparation time70 min/plate
Binding capacity100 μg
RNA ratio28S/18S approx. 2.1
A₂₆₀/A₂₈₀1.90–2.10
Typical RIN (RNA > 9 (cells)

integrity number)≥ 7 (tissue)
Sufficient for:
< 107 cultured cells (centrifugation)
< 30 mg tissue (centrifugation)
saliva

Related products:
Prod #Related prod #
RTN9604RTN9602
RTN9602RTN9604

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

GenElute is a trademark of Sigma-Aldrich Co. LLC