G418 SOLUTION 20 ML

General description

O-2-Amino-2,7-dideoxy-δ-glycero-α-δ-glucoheptopyranosyl[1->4]-O-3-deoxy-4C-methyl-3-[methyl-amino]-β-L-arabinopyranosyl-δ-streptamine, disulfate salt G-418, an aminoglycoside antibiotic, is used as a dominant selection agent in transfection experiments. The antibiotic interferes with the function of 80S ribosomes and blocks protein synthesis in eukaryotic cells. Transfection of neomycin-resistance genes into cells results in resistance to G-418 and enables the cells to grow in culture media containing G-418.

Application

G-418 Solution is used to select eukaryotic cells that are stably transfected with the neomycin resistance gene (neo), and to maintain the phenotype (neo^r) of resistant cells.
G-418 is also used to eliminate contaminating fibroblasts from mixed cultures.

Biochem/physiol Actions

Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.

Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.

Features and Benefits

• Convenient sterile-filtered solution for immediate use – saves valuable time and effort, and avoids potential errors arising from the preparation of sterile working stock solution from G-418 powder.
• Consistent quality and potency – shows no lot-to-lot variation in its ability to select resistant cells.
• Function tested in a cell culture assay - ensures effectiveness.
• Very pure, as indicated by TLC and microbiological assays – allows you to use less antibiotic while producing greater selection pressure. Lack of contaminants means minimal toxicity.
• Cost effective, since the selected colonies are stable.

Contents
Aqueous solution of G-418, 892 μg/mg, filtered through 0.2μm pore size membrane

Quality

Purity: = 98%, as shown by TLC.
Function testing: in cell culture.

Preparation Note

Working concentration: The optimal concentration of G-418 must be determined experimentally and varies with the cell type used.
Active weight: >700μg/mg
Recommended concentration in cell culture: The optimal concentration of G-418 must be determined experimentally; it varies from 100μg/ml to 1mg/ml, depending on the cell type used.
Specific rotation: 104° to 121°, in water (on dry basis, according to USP XXII).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Molecular Weight: 692.71. Empirical Formula: C₂₀H₄₀N₄O₁₀ · 2H₂SO₄. Quality Level: 100. Assay: 98% (TLC). form: solution. mol wt: 692.7 . Da. packaging: pkg of 100 . ml (04727894001 [5 x 20 ml, equivalent to 5 g]), pkg of 20 . mL (04727878001 [equivalent to 1 g]). manufacturer/tradename: Roche. concentration: 50 . mg/mL. technique(s): transfection: suitable. antibiotic activity spectrum: protozoa. Mode of action: protein synthesis | inhibits. storage temp.: 2-8°C. SMILES string: OS(O)(: O): O.OS(O)(: O): O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O. InChI: 1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19. 2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3. 2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+. . /m0../s1. InChI key: UHEPSJJJMTWUCP-NKCAIAFTSA-N. Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H317 - H334. Precautionary Statements: P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311. Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): No data available. Flash Point(C): No data available.