G 418 DISULFATE SALT SOLUTION (C005B-006814)

General description

Chemical structure: aminoglycoside

Application

G418 disulfate is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Biochem/physiol Actions

Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.

Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.

Caution

The product is stable for two years when stored at 2-8°C.

Molecular Weight: 692.71. Empirical Formula: C₂₀H₄₀N₄O₁₀ · 2H₂SO₄. biological source: Micromonospora echinospora. Quality Level: 200. sterility: 0.1 μ. m filtered. product line: BioReagent. form: solution. potency: 50-60 . mg per mL. mol wt: 692.7. packaging: pkg of 10 and 100 . mL. concentration: 50 . mg/mL in H₂O. technique(s): cell culture | mammalian: suitable. impurities: endotoxin, tested. color: colorless. solubility: H₂O: 50 . mg/mL, clear. web metadata keyword.default: Geneticin, selection agent, recombinant protein expression, neo resistance gene selection agent. antibiotic activity spectrum: parasites. Mode of action: protein synthesis | interferes. storage temp.: 2-8°C. SMILES string: OS(O)(: O): O.OS(O)(: O): O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O. InChI: 1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19. 2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3. 2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+. . /m0../s1. InChI key: UHEPSJJJMTWUCP-NKCAIAFTSA-N. Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H317 - H334. Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313. Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.