FURA¡2-AM SUITABLE FOR FLUORESCENCE BIOREAGENT >= 95.0% (HPLC) (C005B-007191)

General description

Fura 2-AM (Fura 2), also known as acetoxy-methyl-ester, is a calcium-specific ratio-metric fluorescent dye. In response to calcium ions, ratio metric dyes like Fura 2 changes their respective excitation or emission spectra. The intracellular calcium concentration is determined by the fluorescence emission ratio or excitation at distinct wavelengths. Fura 2 has an emission peak at 505nm, and the excitation peak ranges between 340-380nm and is the preferred calcium indicator.

Application

Fura 2-AM is a popular probe used to determine dynamic changes in cytosolic free calcium in intact living cells.

Features and Benefits

The major benefits of using the ester form of Fura 2 as Fura 2-AM are:

• It is cell-permeable.
• It can be loaded into cells easily.

Other advantages of using Fura 2-AM over single wavelength dyes is that the ratio signal is independent of :

• The dye concentration.
• Illumination intensity, and
• Optical path length.

Other Notes

Membrane-permeable derivative of Fura-2. Used to load the chelator into cells. Hydrolysed enzymatically in the cell to yield Fura-2 which remains within the cell

Molecular Weight: 1001.85. Empirical Formula: C₄₄H₄₇N₃O₂₄. product line: BioReagent. Quality Level: 100. Assay: ≥. 95.0% (HPLC). form: solid. solubility: DMSO: soluble. fluorescence: λ. _ex . 355 . nm. λ. _em . 495 . nm in 0.1 M Tris pH 8.0, esterase. 10 mM Ca²⁺. suitability: suitable for fluorescence. storage temp.: −. 20°C. SMILES string: CC(: O)OCOC(: O)CN(CC(: O)OCOC(C): O)c1ccc(C)cc1OCCOc2cc3cc(oc3cc2N(CC(: O)OCOC(C): O)CC(: O)OCOC(C): O)-c4ncc(o4)C(: O)OCOC(C): O. InChI: 1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3. InChI key: VPSRLGDRGCKUTK-UHFFFAOYSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.