HUMANKINE FLT3 LIGAND HUMAN RECOMBINANT EXPRESSED IN HEK 293 CELLS CELL CULTURE TESTED

General description

Human FMS-like tyrosine kinase 3 (FLT3) ligand is a homodimer consisting of two short-chain α-helical bundles. The molecular weight of FLT3 ligand is 44 kDa.

Biochem/physiol Actions

FLT3 ligand binds to the cell-bound tyrosine kinase receptor and activates them.

HumanKine FLT3 Ligand is expressed in human HEK 293 cells as a glycosylated monomer with an apparent molecular mass of 24-30 kDa. HumanKine FLT3 Ligand in human HEK 293 cells contributes to glycosylation.FLT3 ligand is a hematopoietic cytokine that regulates the proliferation of early hematopoietic cells. It has been shown to synergize with a variety of cytokines to stimulate the growth and differentiation of early hematopoietic cells. The FLT3 ligand also stimulates the expansion of monocytes and immature dendritic cells, and induces early B cell lineage differentiation and NK cell growth.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of the human acute myeloid leukemia cell line OCI-AML5.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

biological source: human. Quality Level: 100. recombinant: expressed in HEK 293 cells. Assay: ≥. 95% (SDS-PAGE). form: lyophilized powder. potency: ≤. 0.8 . ng/mL EC₅₀. quality: endotoxin tested. mol wt: monomer 24-30 . kDa (glycosylated). packaging: pkg of 10 . μ. g. storage condition: avoid repeated freeze/thaw cycles. technique(s): cell culture | mammalian: suitable. impurities: ≤. 1 . EU/mg. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.