FIBROBLAST GROWTH FACTOR-BASIC FROM BOVINE PITUITARY FGF-BASIC SUITABLE FOR CELL CULTURE

General description

The fibroblast growth factor-2 (FGF2) gene is predominantly expressed in uterus, cornea and vascular endothelial cells. The FGF2 gene is mapped to cattle chromosome 17.

Application

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor-Basic from bovine pituitary has been used:

• In animal and human cell culture
• In neuronal induction of adipose and bone marrow-derived rat mesenchymal stem cells
• In the generation of directly reprogramming fibroblast
• In human glioma cell culture

Biochem/physiol Actions

Fibroblast growth factor-2 (FGF2) stimulates cell proliferation, differentiation and migration. It is also known to participate in angiogenesis and carcinogenesis. During the estrous cycle, FGF2 expression is found to be maximum in porcine conceptuses and endometrium. Upregulation of this gene is also observed in pregnancy during peri-implantation. FGF2 is involved in embryogenesis, tissue repair and other metabolic pathways.

Physical form

Lyophilized from a 0.2 μm filtered solution in sodium phosphate and sodium chloride containing 0.1 mg bovine serum albumin

Preparation Note

Purified glands by a modification of the method of Gospodarowicz, et al.

Analysis Note

The proliferative activity is tested in culture using fetal bovine heart endothelial cells.

biological source: bovine pituitary glands. Quality Level: 200. Assay: ~90% (SDS-PAGE and N-terminal analysis). form: lyophilized powder. potency: 0.03-3.0 . ng/mL ED₅₀/EC₅₀. quality: endotoxin tested. mol wt: 16.4 . kDa. packaging: pkg of 1 . μ. g. storage condition: avoid repeated freeze/thaw cycles. technique(s): cell culture | mammalian: suitable. impurities: <. 0.5 . EU/vial. color: white. UniProt accession no.: P03969. storage temp.: −. 20°C. Gene Information: bovine ... FGF2(281161). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).