FAS LIGAND/TNFSF6 MOUSE RECOMBINANT

General description

FASLG (Fas ligand) acts as a ligand for Fas receptor, and is a major protein involved in programmed cell death, apoptosis. Soluble Fas (sFAS) is usually detected in plasma prior to apoptosis.

Application

Fas Ligand (FASLG) from mouse has been used for-

• the induction of apoptosis in PC12 cells and
• the induction of migration in BV-2 murine microglial cells.

Biochem/physiol Actions

FASLG (Fas ligand) and Fas receptor constitute the basic elements in apoptosis. Interaction of FASLG with Fas receptor leads to activation of caspase-8. This caspase in turn leads to activation of effector caspases such as caspase-3, -6 and -7. This cascade results in the hydrolysis of nuclear and cytoplasmic components. Expression of FASLG is induced by nuclear factor-κB (NFκB). NFκB/FASLG pathway facilitates the suppression of p,p′-DDT (dichlorodiphenoxytrichloroethane)-induced cell toxicity by vitamin C and E. In CD4⁺ T cells, this protein is expressed on stimulus by T-cell receptor (TCR), both during normal and pathological conditions, such as alcohol exposure.

Fas ligand, a protein belonging to the tumor necrosis factor (TNF) family of cytokines, induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1).

Other Notes

Mouse Fas Ligand, N-terminal 6X histidine-tagged, encodes amino acid residues 132-279.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

Measured by its ability to induce apoptosis in Jurkat cells.

recombinant: expressed in mouse NSO cells. Quality Level: 100. Assay: >. 95% (SDS-PAGE). form: lyophilized powder. mol wt: monomer calculated mol wt ~18 . kDa, 28-32 . kDa by SDS-PAGE. impurities: endotoxin, tested. UniProt accession no.: P41047. storage temp.: −. 20°C. Gene Information: mouse ... Fasl(14103). Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.