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f9 embryo teratocarcinoma (c15-1310-270)

F9 EMBRYO TERATOCARCINOMA (C005B-234440)

Catalog No.
C005B-234440
Manufacturer No.
85061803-1VL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
VI

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Cell Line Origin Mouse 129 embryo teratocarcinoma Cell Line Description Derived from testicular carcinoma. F9 undergo very limited differentiation under normal conditions but can be induced to differentiate into parietal endoderm in the presence of

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Cell Line Origin

Mouse 129 embryo teratocarcinoma

Cell Line Description

Derived from testicular carcinoma. F9 undergo very limited differentiation under normal conditions but can be induced to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cAMP.

Application

Differentiation studies

Culture Medium

DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum (FBS). Pre-soak flasks in 0.1% gelatin for over 2 hours and wash with PBS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2, 37°C. Upon resuscitation, cells shed large quantities of debris, leave for 2 days before subculture. Please note: Culture v

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source: mouse embryo. growth mode: Adherent. karyotype: Not specified. morphology: Not specified. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.
UPC:
51111810
Condition:
New
HazmatClass:
No
MPN:
85061803-1VL
Temperature Control Device:
Yes

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