ETHYLENE GLYCOL-BIS(2-AMINOETHYLETHER)-N N N' N'-TETRAACETIC ACID (C005B-056220)

Application

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid has been used:

• in Dulbecco′s phosphate-buffered saline (DPBS) to induce the weakening of cell-to-cell junctions
• as an extracellular calcium chelator to study the role of calcium in axonal mitochondria degeneration
• as a component in lysis buffer for lysing mouse mucosal cells for western blot analysis

A chelating agent useful for the determination of calcium in the presence of magnesium.

Biochem/physiol Actions

Ethylene glycol tetraacetic acid (EGTA) can chelate calcium (Ca²⁺) at a ratio of 1:1. It serves as an anti-coagulant when dissolved at 1 g per 100 ml of blood. EGTA can prevent the vesicular release in the nanodomain of single Ca²⁺ channels. This metal chelator can inhibit the activity of pseudolysin.

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid A is a chelating agent used for the determination of calcium in the presence of magnesium.

Molecular Weight: 380.35. Empirical Formula: C₁₄H₂₄N₂O₁₀. linearFormula: [-CH₂OCH₂CH₂N(CH₂CO₂H)₂]₂. grade: for molecular biology. Quality Level: 200. Assay: ≥. 97.0%. form: powder. reaction suitability: reagent type: chelator. loss: ≤. 1.0% loss on drying. mp: 241 . °C (dec.) (lit.). foreign activity: DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected. SMILES string: OC(: O)CN(CCOCCOCCN(CC(O): O)CC(O): O)CC(O): O. InChI: 1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24). InChI key: DEFVIWRASFVYLL-UHFFFAOYSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 2. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).